Role of aryl hydrocarbon receptor (AHR) in overall retinoid metabolism: Response comparisons to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) exposure between wild-type and AHR knockout mice

Young adult wild-type and aryl hydrocarbon receptor knockout (AHRKO) mice of both sexes the C57BL/6J background were exposed to 10 weekly oral doses 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD; total dose 200 ?g/kg bw) further characterize observed impacts AHR as well TCDD on retinoid system. Unexposed AHRKO harboured heavier kidneys, lighter livers lower serum all-trans retinoic acid (ATRA) retinol (REOH) concentrations than mice. Results from present study also point a role for murine in control circulating REOH ATRA concentrations. In mice, elevated liver weight reduced thymus weight, drastically hepatic 9-cis-4-oxo-13,14-dihydro-retinoic (CORA) retinyl palmitate (REPA). female increased concentration renal Renal CORA substantially diminished male exclusively following TCDD-exposure, with similar tendency serum. contrast, did not affect any these toxicity or system parameters Finally, distinct sex difference occurred kidney all analysed forms. Together, results strengthen evidence mandatory TCDD-induced disruption, suggest that previously reported accumulation several forms is line-specific phenomenon. Our data support participation development